Study of morphometric characteristics of collagen fibers in early terms of subcutaneous implantation of porous fibrous matrix

Authors

  • Андрій Володимиров Пантус
  • Микола Михайлович Рожко
  • Микола Миколаєвич Багрій
  • Наталія Євгеніївна Ковальчук
  • рина Романівна Ярмошук І
  • Василь Васильович Грекуляк

DOI:

https://doi.org/10.33295/1992-576X-2019-3-68

Keywords:

biopolymer, bioimplant, collagenfibers

Abstract

Resume. The problem facing tissue engineering is to optimize the selection, reproduction and differentiation of cells, to construct matrices or delivery systems, contributing to the maintenance, coordination of tissue regeneration in three dimensions. Currently the interest in biopolymers in bioengineering and medicine is increasing progressively. The materials used in tissue engineering are expected to have some special features and supply engineer and microengineer constructions with characteristics that living tissues have, as follows: the ability of self-repair; the ability of changing anatomy and properties in response to environmental factors.
The purpose of the study is to experimentally evaluate the nature of the collagen substrate development in the early terms of subcutaneous implantation of a biopolymer fibrous matrix. The research was carried out on 20 lab animals (rabbits), which were divided into two groups. The first group: surgical intervention was performed to 10 animals, which included the creation of the pocket in the subcutaneous adipose tissue and suturing. The second group: the implantation of the biopolymer matrix into the area between the shoulder blades was performed to 10 animals. The month after the atrix with the underlying tissues was removed. It was divided into 25 parts for histological examination. 9 segments were chosen for analysis: the one from centrally located area, four segments from precentral and four ones from peripheral zones. The histological slides were examined lightoptically with the help of the microscope «Leica DME@ with different magnifications. The morphometric features were analyzed using the system for taking microscopic pictures of histological samples (microscope «Leica DME» and digital camera «Nikon P 5100») and by using the program Image- Tool 2.0 for Windows. The research were conducted at the Department of Pathomorphology and Legal Medicine of Ivano-Frankivsk National Medical University. Statistical analysis was performed with the aid of PC programs Microsoft Exel and Statistica 5.5 (Multiple Regression) using the methods of variation statistic and correlation. On the basis of the performed research it was established that the growth and formation of collagen fiber occurred through the entire thickness of the fibrous polymer matrix in three mutually perpendicular directions. This fact confirms the framework function of the polymer mesh we synthesized. The obtained results indicate the absence of both acute and chronic reactive inflammatory infiltration, as well as acute and chronic rejection of the implant as a foreign substance in the implanted part of the animal. The fibrous matrix created by us because of its hygroscopicity and porosity creates a unique bridge for germination of tissues and the formation of a collagen matrix in a three-dimensional space.
Key words: biopolymer, bioimplant, collagenfibers.

Author Biographies

Андрій Володимиров Пантус

Пантус Андрій Володимирович – канд. мед. наук,
доцент кафедри хірургічної стоматології ДВНЗ «Івано-Франківський національний медичний університет».
Адреса: вул. Галицька, 2, м. Івано-Франківськ, 76018. Тел.: (066) 102-68-56. E-mail: kovalchuk-natalja@ukr.net.

Микола Михайлович Рожко

Рожко Микола Михайлович – д-р мед. наук, професор, Заслужений діяч науки і техніки України,
ректор ДВНЗ «Івано-Франківський національний медичний університет».
Адреса: вул. Галицька, 2, м. Івано-Франківськ, 76018. Тел.: 0-342 78-41-94. E-mail: rector@ifnmu.edu.ua.

Микола Миколаєвич Багрій

Багрій Микола Миколаєвич – канд. мед. наук,
доцент кафедри патоморфології та судової медицини ДВНЗ «Івано-Франківський національний медичний університет».
Адреса: вул. Галицька, 2, м. Івано-Франківськ, 76018. Тел.: (050) 733-48-72. E-mail: Pathomorfology@ifnmu.edu.ua.

Наталія Євгеніївна Ковальчук

Ковальчук Наталія Євгеніївна – канд. мед. наук,
асистент кафедри клінічної фармакології і фармакотерапії ДВНЗ «Івано-Франківський національний медичний університет».
Адреса: вул. Галицька, 2, м. Івано-Франківськ, 76018. Тел.: (066) 568-60-20. E-mail: kovalchuk-natalja@ukr.net.

рина Романівна Ярмошук І

Ярмошук Ірина Романівна – канд. мед. наук,
асистент кафедри стоматології ННІ ПО ДВНЗ «Івано-Франківський національний медичний університет».
Адреса: вул. Галицька, 2, м. Івано-Франківськ, 76018. E-mail: zlatoslava2@ukr.net.

Василь Васильович Грекуляк

Грекуляк Василь Васильович – асистент кафедри хірургічної стоматології
ДВНЗ «Івано-Франківський національний медичний університет».
Адреса: вул. Галицька, 2, м. Івано-Франківськ, 76018. Тел.: (050) 966-47-39. E-mail: bezvywkin23@gmail.com.

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Published

2019-10-10

Issue

Section

IMPLANTOLOGY