Analysis of the dominant microbial associations of the cavity of the mouth and especially their sensitivity to antibacterial drugs
DOI:
https://doi.org/10.33295/1992-576X-2018-5-37-39Keywords:
isolates of antibiotic resistance, pathogenic microorganisms, conditionally pathogenic microorganisms, antibacterial activity, antimycotic activityAbstract
Actuality. Conditionally pathogenic bacteria play a significant role in the development of inflammatory diseases. The growing tendency towards the formation of antibiotic resistant microorganisms causes the relevance of monitoring of microorganisms' sensitivity to antimicrobial drugs and the development of new approaches to antimicrobial therapy.
Aim: to study the dominant representatives of microbiotis of the oral cavity, to find their sensitivity to antibacterial drugs. Materials and methods. For studying microbial associations a bacteriological study of pathological material from the inflammatory cell with the use of differential diagnostic nutrient media and the subsequent identification of isolated cultures based on morphological, tinctorial and biochemical properties was performed. Determination of antibiotic susceptibility of isolates was carried out by diffusion method, to antiseptics and phytopreparations – by diffusion in agar.
Results. It was established that in 43.60 % of cases from the cell of the inflammatory process bacteria of the genus Staphylococcus were sown; in 27.6 % of patients, bacteria of the genus Streptococcus belonging to the S. pyogenes, S. agalactiae, S. viridans, S. mutans, S. pneumoniae species were isolated; In 20 % of the patients, the bacteria of the Enterobacteriaceae family were isolated. A high degree of antibiotic resistance of microorganisms isolated from cells of the inflammatory process was demonstrated: 65 % of isolates were resistant to at least 7 antibiotics. 3 isolates of Klebsiella rhinoskleromatis and one Streptococcus pyogenes were resistant, more than 20 antibiotics. All isolates were resistant to ampicillin, erythromycin, tetracycline. It was found that the most effective against the isolates were fluoroquinolones and cefoperazone/sulbactam. From antiseptics, dexan showed a wide range of antimicrobial activity, but the dose we applied did not affect Candida spp. The highest anti-staphylococcal action was shown by the antibacterial drug Dioxidine. The antimicrobial effect on the fungi of the genus Candida is found only with the use of chlorhexidine.
Conclusions. The established regularities determine the relevance of the individual approach to the selection of antibiotic therapy withthe definition of antibiotic susceptibility of pathogens of the inflammatory process and the development of a comprehensive approach to the correction of opportunistic microbiotis.
Key words: isolates of antibiotic resistance, pathogenic microorganisms, conditionally pathogenic microorganisms, antibacterial activity, antimycotic activity.
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